Invitrogen™ SDF-1 alpha/CXCL12 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMCXCL12

Product Code. 15445253

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α. Principle of the method The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P40224
• Assay Sensitivity: 3 pg/mL
• Description: SDF-1 alpha CXCL12 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of SDF-1 alpha CXCL12 in Samples Using a Microplate Rea
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Pbsf,Scyb12,Sdf1,Tlsf,Tpar1
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse SDF-1 alpha precoated 96-well strip plate, lyophilized recombinant mouse SDF-1 alpha standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 9.38-600 pg/mL
• Calibration Range: 3pg/mL to 600pg/mL
• Detectable Analytes: SDF-1 alpha CXCL12, mouse SDF-1
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 20315
• Host Species: Mouse
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
• Test Time: 1 hr. 20 min.