Eurofins DiscoverX SGK1 (S422D) Protein, Unactive

Unactive, N-truncated (missing N-terminal 59 amino acids), N-terminal 6His-tagged, human SGK1 fusion protein, containing an S422D mutation, for use in Kinase Assays.

Brand:  Eurofins DiscoverX 14-332

Product Code. 17320144

Description

The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorlation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress.

GenBank AF153609

Specifications

• Formulation: mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 0.1 mM EGTA, 150 mM NaCl, 0.03% Brij-35, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol, 270 mM sucrose. Frozen solution.
• Quantity: 25 μg
• Recombinant: Recombinant
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Purity or Quality Grade: ≥73%

• Molecular Weight (g/mol): 45.7 kDa
• Species: Human
• Protein Tag: N-terminal 6His
• Protein Form: Truncated
• Protein: SGK1 (S422D), unactive