SPT6 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA557634

Product Code. 15715531

Description

Immunogen sequence: RTTYIKRVIA HPSFHNINFK QAEKMMETMD QGDVIIRPSS KGENHLTVTW KVSDGIYQHV DVREEGKENA FSLGATLWIN SEEFEDLDEI VARYVQPM Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 100%.

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Specifications

• Antigen: SPT6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SUPT6H
• Gene Alias: 5131400N11Rik; AI132449; emb-5; Histone chaperone suppressor of Ty6; hSPT6; Kiaa0162; MGC87943; mKIAA0162; SPT6; SPT6 homolog, histone chaperone; SPT6H; suppressor of Ty 6; suppressor of Ty 6 homolog; suppressor of Ty 6 homolog (S. cerevisiae); Supt6; Supt6h; Tat-cotransactivator 2 protein; tat-CT2 protein; Transcription elongation factor SPT6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6830
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q7KZ85
• Gene Symbols: SUPT6H
• Immunogen: Recombinant protein corresponding to Human SPT6
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Research Use Only