IBA Lifesciences Strep-Tactin™XT Buffer Set

Strep-Tactin™XT protein purification buffer set with wash, elution and regeneration buffer

Brand:  IBA Lifesciences 2-1043-000

Product Code. 17044437

Description

Strep-Tactin™XT Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin™XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin™XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from Strep-Tactin™, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin™ cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin™XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin™XT after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. Please note that the Strep-Tactin™XT Buffer Set is not applicable for Strep-Tactin™.

Specifications

• Concentration: 10 x concentrated
• Kit Contents: 100 mL 10 x Buffer W, 25 mL 10 x Buffer BXT, 250 mL Buffer XT-R
• Shipping Condition: room temperature
• Form: Reagent Solution

Safety and Handling

Product Identifier

• 1SET Strep-TactinXT Purification Buffer Set

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.