Cytiva StrepTrap™ HP Prepacked Columns

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.

Brand:  Cytiva 28-9075-48

Product Code. 11540654

Description

• Prepacked with StrepTactin Sepharose High Performance
• Binding capacity Approx. 6 mg Strep II-tagged protein/ml medium
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Generated with 0.5M NaOH
• 1 and 5mL columns

Media

• Ligand: Streptactin
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: >7

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Product Type: StrepTactin Sepharose HP prepacked column
• Diameter (English) Inner: 0.62 in.
• Quantity: 5 x 5mL
• Height (Metric): 25mm
• Material: Polypropylene
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Content And Storage: 4° to 8°C
• For Use With (Application): Purification of Strep (II) -tagged recombinant Proteins by affinity chromato graphy

• Bed Volume: 5mL
• Diameter (Metric) Inner: 16mm
• Height (English): 0.98 in.
• Flow Rate: <20 mL/min.
• Ligand Type: StrepTactin (∼5mg/mL medium)
• pH Range: 7
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Ion Exchange Column

Safety and Handling

Product Identifier

• StrepTrap HP, 5 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Ethanol