Thermo Scientific™ Tango™ CCR1-bla U2OS DA Assay Kit

The Tango™ CCR1-bla U2OS DA cells contain the human Chemokine (C-C Motif) Receptor 1 (CCR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1793

Product Code. 10003043

Description

The Tango™ CCR1-bla U2OS DA cells contain the human Chemokine (C-C Motif) Receptor 1 (CCR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CCR1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ CCR1-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

• Accession Number: NM_001295
• Cell State: Division-Arrested Cells
• Cytoplasmic Tail: Tail from GRPR GPCR
• Detection Method: Fluorescence
• Gene Symbol: CCR1
• Population Status: Single Cell Clone Origin
• Protein Family: GPCR (G-Protein Coupled Receptors)
• Readout: End Point
• Sample Type: U2OS
• Type: Division-Arrested Cells Assay Kit
• System Type: Tango™

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • Tango™ CCR1-bla U2OS DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • Tango™ CCR1-bla U2OS DA Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The Tango™ CCR1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Description: Tango™ CCR1-bla U2OS DA Assay Kit, 1 Kit, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, target and lead identification and validation
• Includes: Tango™ CCR1-bla U2OS DA assay kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: Tango™ CCR1-bla U2OS DA cells, LiveBLAzer™-FRET B/G loading kit, 70μg, solution D, Tango™ CCR1-bla U2OS DA assay protocol, LiveBLAzer™-FRET B/G loading protocol, certificate of analysis
• Product Line: GeneBLAzer™
• Quantity: 1 Kit
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: CCR1
• Cell Line: U2OS
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.