Thermo Scientific™ Tango™ CXCR3-bla U2OS Cells

The Tango™ CXCR3-bla U2OS cells contain the human chemokine (C-X-C motif) receptor 3 (CXCR3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1829

Product Code. 10471215

Description

This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CXCR3-bla U2OS cells are functionally validated for Z' and EC50 concentrations of I-TAC (CXCL11). In addition, Tango™ CXCR3-bla U2OS cells have been tested for assay performance under variable conditions. This data are found in the Validation & Assay Performance Summary.

Specifications

• Accession Number: NM_001504
• Cell State: Dividing Cells
• Cytoplasmic Tail: Native Tail
• For Use With (Application): FRET
• Human Protein Class: G-Protein Coupled Receptor (GPCR)
• Product Line: GeneBLAzer™, Tango™
• Readout: End Point
• Type: U2OS Cell
• System Type: Tango™

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: Contents:
  K1829 Tango™ CXCR3-bla U2OS cells
  (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
  Includes:
  • Tango™ CXCR3-bla U2OS cells
  • Tango™ CXCR3-bla U2OS Assay Protocol
  • Certificate of Analysis
  
  Storage:
  The Tango™ CXCR3-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: CXCR3
• Population Status: Single Cell Clone Origin
• Quantity: 8 x 10⁶ Cells
• Target Entry: CXCR3
• Cell Line: U2OS
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.