Thermo Scientific™ Tango™ GPR119-bla U2OS Cells

The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1777

Product Code. 10522304

Description

The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR119-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Specifications

• Accession Number: NM_178471.1
• Cell State: Dividing Cells
• Cytoplasmic Tail: Native Tail
• For Use With (Application): GPCRs
• Includes: GPR119-bla U2OS cells, GPR119-bla U2OS Assay Protocol, Certificate of Analysis
• Product Line: GeneBLAzer™
• Quantity: 8 x 10⁶ Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Dividing Cells
• System Type: Tango™

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
  
  Includes:
  • Tango™ GPR119-bla U2OS cells
  • Tango™ GPR119-bla U2OS Assay Protocol
  • Certificate of Analysis
  
  The Tango™ GPR119-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: GPR119
• Population Status: Single Cell Clone Origin
• Protein Family: GPCR (G-Protein Coupled Receptors)
• Readout: End Point
• Target Entry: GPR119
• Cell Line: U2OS
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.