Thermo Scientific™ Tango™ M1-bla U2OS Cells

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1861

Product Code. 10217274

Description

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ M1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of oxotremorine M. In addition, Tango™ M1-bla U2OS cells have been tested for assay performance under variable conditions.

Specifications

• Accession Number: NM_000738
• Cell State: Dividing Cells
• Cytoplasmic Tail: Native Tail
• For Use With (Application): FRET
• Population Status: Single Cell Clone Origin
• Quantity: 8 x 10⁶ Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Dividing Cells
• System Type: Tango™

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
• Detection Method: Fluorescence
• Human Protein Class: G-Protein Coupled Receptor (GPCR)
• Product Line: Tango™
• Readout: End Point
• Target Entry: M1
• Cell Line: U2OS
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.