Thermo Scientific™ Tango™ OPRL1-bla U2OS Cells

The Tango™ OPRL1-bla U2OS cells contain the human Opioid Receptor-Like 1 (OPRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1786

Product Code. 10448073

Description

The Tango™ OPRL1-bla U2OS cells contain the human Opioid Receptor-Like 1 (OPRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ OPRL1-bla U2OS cells have been functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, Tango™ OPRL1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Specifications

• Accession Number: NM_000913
• Cell State: Dividing Cells
• Cytoplasmic Tail: Tail from AVPR2 GPCR
• For Use With (Application): FRET
• Human Protein Class: G-Protein Coupled Receptor (GPCR)
• Population Status: Single Cell Clone Origin
• Protein Family: GPCR (G-Protein Coupled Receptors)
• Readout: End Point
• Target Entry: OPRL1
• Cell Line: U2OS
• Druggable Target: GPCR

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
  
  Includes:
  • Tango™ OPRL1-bla U2OS cells
  • Tango™ OPRL1-bla U2OS Assay Protocol
  • Certificate of Analysis
  
  The Tango™ OPRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: OPRL1
• Includes: OPRL1-bla U2OS cells, OPRL1-bla U2OS Assay Protocol, Certificate of Analysis
• Product Line: GeneBLAzer™
• Quantity: 8 x 10⁶ Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Type: U2OS Cell
• System Type: Tango™

For Research Use Only. Not for use in diagnostic procedures.