Thermo Scientific™ Tango™ OPRM1-bla U2OS DA Assay Kit

The Tango™ OPRM1-bla U2OS DA (Division Arrested) cells contain the human Opioid Receptor Mu 1 (OPRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1599

Product Code. 10521824

Description

The Tango™ OPRM1-bla U2OS DA (Division Arrested) cells contain the human Opioid Receptor Mu 1 (OPRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ OPRM1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of DAMGO. In addition, Tango™ OPRM1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

• Accession Number: NM_000914
• Cell State: Division-Arrested Cells
• Cytoplasmic Tail: Tail from AVPR2 GPCR
• Detection Method: Fluorescence
• Gene Symbol: OPRM1
• Population Status: Single Cell Clone Origin
• Quantity: 1 Plate
• Sample Type: U2OS
• Type: Division-Arrested Cells Assay Kit
• System Type: Tango™

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • Tango™ OPRM1-bla U2OS DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • Tango™ OPRM1-bla U2OS Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The Tango™ OPRM1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Description: Tango™ OPRM1-bla U2OS DA Assay Kit, 1 Plate, ∽8,000,000 cells/vial Quantity of Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Includes: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: Tango™ OPRM1-bla U2OS DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, Tango™ OPRM1-bla U2OS Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
• Product Line: Tango™
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: OPRM1
• Cell Line: U2OS
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.