Thermo Scientific™ Tango™ SSTR5-bla U2OS DA Assay Kit

The Tango™ SSTR5-bla U2OS cells contain the human Somatostatin Receptor 5 (SSTR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1573

Product Code. 10676414

Description

The Tango™ SSTR5-bla U2OS cells contain the human Somatostatin Receptor 5 (SSTR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ SSTR5-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ SSTR5-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

• Accession Number: NM_001053
• Cell State: Division-Arrested Cells
• Cytoplasmic Tail: Tail from AVPR2 GPCR
• For Use With (Application): GPCRs
• Population Status: Single Cell Clone Origin
• Quantity: 8 x 10⁶ Cells
• Target Entry: SSTR5
• Cell Line: U2OS
• Druggable Target: GPCR

• Assay Entry: Cell-based beta-arrestin recruitment
• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • Tango™ OPRK1-bla U2OS DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • Tango™ OPRK1-bla U2OS DA Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The Tango™ OPRK1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: SSTR5
• Product Line: Tango™
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Division-Arrested Cells Assay Kit
• System Type: Tango™

For Research Use Only. Not for use in diagnostic procedures.