Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit

TaqMan™ Protein Assays Core Reagents Base Kit

Brand:  Applied Biosystems™ 4448592

Product Code. 12047799

Description

TaqMan™ Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.

Specifications

• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
• For Use With (Application): Real Time PCR (qPCR), Stem Cell Analysis
• Detection Method: Primer-Probe Detection
• Product Type: Protein Assays Core Reagents Base Kit
• Hot Start: No
• Passive Reference Dye: ROX (Pre-mixed)
• Primer Probe Compatibility: TaqMan™
• Reaction Speed: Fast
• Target Specificity: Not Target-Specific

• Product Line: TaqMan™
• Content And Storage: The TaqMan™ Protein Assays Core Reagents Base Kit contains:
  
  DNA Ligase, 500X (1 × 50 μL)
  Ligase Dilution Buffer, 1X (1 × 15 mL)
  Ligation Reaction Buffer, 20X (1 × 4 mL)
  Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 5 mL)
  Protease, 100X (1 × 50 μL)
  Universal PCR Assay, 20X (1 × 600 μL)
  Store all components at -15 to -25°C.
• Label or Dye: FAM
• Sample Type: Tissue, Cells
• No. of Reactions: 500
• Polymerase: AmpliTaq™ Fast DNA Polymerase
• PCR Method: qPCR Protein Quantification
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.