Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

Brand:  Thermo Scientific™ 89822

Product Code. 15265743

Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Description

• An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
• All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
• This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
• Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

• Detergent in Tris buffer with Lysozyme and Universal Nuclease
• Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
• Recovery of large molecular weight proteins (high MW proteins)
• Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
• More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications

Specifications

• Formulation: Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
• Product Line: B-PER™
• Product Type: Bacterial Protein Extraction Reagent

• Content And Storage: Store at 4°C.
• Quantity: 500 mL
• Volume (Metric): 500 mL

Safety and Handling

Product Identifier

• BPER Complete Bacterial Protein Extraction Reagent

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy

For Research Use Only. Not for use in diagnostic procedures.