Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells

Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products

Product Code	Brand	No. of Reactions
17176458	Invitrogen™ K8040-10	10 Reactions
17186458	Invitrogen™ K8040-20	20 Reactions

Description

• The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb).
• The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™ SuperFi™ Green PCR Master Mix, which is included in the kit.
• Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).
• ccdB gene for positive selection
• EcoR I site flanking the PCR product insertion site for easy excision of inserts
• Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
• T7 promoter/priming site for in vitro RNA transcription and sequencing
• T7, T3, and M13 forward and reverse primer sites for sequencing
• TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
• Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
• PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes

Specifications

• Bacterial or Yeast Strain: Not Included
• Cloning Method: Zero Blunt TOPO
• Format: Kit
• Product Line: TOPO™
• Promoter: T3, T7
• Vector: pCR-XL-2-TOPO

• Antibiotic Resistance Bacterial: Ampicillin (AmpR), Kanamycin (KanR)
• Content And Storage: Box 1: Store at -5 to -30°C.
  • pCR XL-2-TOPO vector
  • Salt Solution
  • T3 Primer
  • T7 Primer
  • XL Control PCR Template
  • XL Control PCR Primers
  • Platinum SuperFi Green PCR Master Mix
  • SuperFi GC Enhancer
  • Nuclease-free water
  
  Box 2: Store at room temperature.
  • Gel Solubilization Buffer (L3)
  • Binding Buffer (B2)
  • Wash Buffer (W1)
  • Elution Buffer (E1)
  • PureLink Clean-up Spin Columns (in Wash Tubes)
  • PureLink Recovery/Elution Tubes
  
  
• For Use With (Application): PCR Cloning
• Product Type: Cloning Kit
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

For Research Use Only. Not for use in diagnostic procedures.