Gibco™ William's E Medium, no glutamine

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells.

Brand:  Gibco™ 12551032

Product Code. 12599059

Description

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

This William's E Medium is modified as follows:

• With: Phenol Red
• Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Dual-Site cGMP Manufacturing

Gibco™ William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Form: Liquid
• With Additives: Phenol Red, Sodium Pyruvate
• For Use With (Application): Hepatocytes and hepatic models, mammalian cell culture, pharma and biopharma, primary cell culture
• Quantity: 500 mL

• Culture Type: Mammalian Cell Culture
• Product Type: William's E Medium
• Without Additives: No Glutamine, No HEPES
• Green Features: Sustainable packaging
• Shipping Condition: Room Temperature