PCR Equipment and Supplies
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PCR Equipment and Supplies
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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
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GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Primer-probe |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Frozen, Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | • Express Lysis Solution (room temperature or 4°C) • Express ezDNase (–20°C) • SuperScript IV VILO Master Mix (–20°C) • SuperScript IV VILO No RT Control (–20°C) • TaqMan Fast Advanced Master Mix (4°C) |
Form | Liquid |
Isolation Technology | Direct cell lysis |
Kit Type | Direct Cell Lysis RT-qPCR |
Green Features | Less hazardous, less waste |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | Yes |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Target | cDNA |
Format | Kit |
Sample Type | Cultured mammalian cells |
Reaction Speed | Fast |
Label or Dye | ROX |
Purification Target | RNA |
Shelf Life | 1 Year |
Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
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The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
Shipping Condition | Dry Ice |
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Sample Type | Cells |
Reaction Speed | Fast or Standard |
Purification Time | 7 min. |
Green Features | Fewer resources used and less waste, less hazardous |
Starting Material | 1–10 Cells |
For Use With (Application) | RT-PCR |
Product Line | Ambion™, Cells-to-CT™ |
Detection Method | Primer-probe |
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
PCR Method | 2-step RT-qPCR |
Detection Method | SYBR |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
---|---|
Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, Power SYBR™ Green |
Detection Method | SYBR |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Shipping Condition | Wet Ice |
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Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Run Time | Standard |
Product Line | RNA-to-CT™, SYBR™ |
TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
Polymerase | AmpliTaq Gold DNA Polymerase |
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Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
Product Type | One-Step RT-PCR Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | RNA-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit
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First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
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Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | LD (Low DNA) |
Reverse Transcriptase | M-MLV |
Purification Time | 5 min. |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 20X |
For Use With (Application) | RT-PCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cells |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Hot Start | Built-In Hot Start |
Product Line | Cells-to-CT™, SYBR™ |
Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit
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The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.
Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit
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The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | M-MLV |
Green Features | Fewer resources used and less waste, Less hazardous |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | DNA Polymerase |
Format | Tube |
Product Type | Gene Expression Cells-to-C Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell |
Reaction Speed | Standard |
Product Line | Ambion™, Cells-to-CT™, TaqMan™ |