Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

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Specifications

Cut Site	CTCTTC(1/4)^
Content And Storage	Store at -20°C.
Form	Liquid
Enzyme	Eam1104I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity	150 μL
Type IIS RE	Yes
Incubator Temperature	37°C
Concentration	20 U/μL
For Use With (Application)	In vitro Transcription
Inactivation Temperature	65°C
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Research Category	Drug Development
Compatible Buffer	10X Tango Buffer
Optimal Reaction Temperature	37°C
Product Line	TheraPure™

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

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Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

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Thermo Scientific™ FastDigest NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nde I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

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Thermo Scientific™ FastDigest SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	SacI
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	3' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NheI

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nhe I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Eco31I
Methylation Sensitivity	CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nco I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Bcu I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

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Thermo Scientific™ FastDigest Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Eco32I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	Blunt
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

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Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)

The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	HpyF3I (DdeI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Restriction Enzymes

Restriction Enzymes

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