Buffers and Diluents

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Invitrogen™ NuPAGE™ Antioxidant

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ DNA Gel Loading Dye (6X)

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Cytiva Pharmalyte™ Carrier Ampholytes

Cytiva Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Thermo Scientific™ SuperBlock™ Blocking Buffer

Thermo Scientific™ SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)

Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)

Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels.

Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)

Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)

Ideal for native running conditions with NuPAGE Tris-Acetate gels

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Invitrogen™ NativePAGE™ Sample Buffer (4X)

Invitrogen™ NativePAGE™ Sample Buffer (4X)

A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System

Invitrogen™ Bolt™ Antioxidant

Invitrogen™ Bolt™ Antioxidant

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,<i>Includes LC5300, LC5310, LC5311</i>

Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311

Optimized for using with Novex™ pH 3-10 IEF gels

Invitrogen™ NativePAGE™ Running Buffer Kit

Invitrogen™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X)

Invitrogen™ eBioscience™ IC Fixation Buffer

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

Invitrogen™ Digitonin (5%)

Invitrogen™ Digitonin (5%)

Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.

Cytiva ExcelGel SDS Buffer Strips

Cytiva ExcelGel SDS Buffer Strips

Semi dry precast buffer strips for horizontal SDS electrophoresis.  Cytiva Lifescience™ ExcelGel SDS Buffer Strips are designed for use with ExcelGel Precast Gels.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent

For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.

Thermo Scientific™ Poly-HRP Dilution Buffer

Thermo Scientific™ Poly-HRP Dilution Buffer

Specifically designed to optimize performance of Poly-HRP Streptavidin

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