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Buffers and Standards

Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

  • Red: pH 4.0
  • Yellow: pH 7.0
  • Blue: pH 10.0

 

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Kjeldahl Buffers and Reagents (13)
Quality Control and Proficiency Samples (12)
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1 – 15 of 8,636 results
1

Buffer Solution pH4.00 (Phthalate), NIST Standard Solution ready to use for pH measurement, Fisher Chemical™

MDL Number: 217672

Promotions are available
EDGE
MDL Number 217672
2

Thermo Scientific™ Orion™ pH Buffer Bottles

Perform accurate, reproducible pH calibrations with these color-coded buffer bottles.

EDGE
For Use With (Equipment) pH Meter and Electrode
Traceability to NIST Yes
Physical Form Liquid
Chemical Name or Material pH Buffer, bottles
3

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

Promotions are available
EDGE
Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
4

Thermo Scientific Chemicals Bradford Dye Reagent, Ready-to-Use soln.

Suitable for protein concentration determination (100 to 1500μg)

Promotions are available
Name Note Ready-to-Use soln.
Health Hazard 3 P234-P260-P264b-P270-P271-P280-P303+P361+P353-P304+P340-P305+P351+P338-P310-P330-P331-P363-P390-P501c
Health Hazard 2 GHS H Statement
H314-H318-H371
Causes severe skin burns and eye damage.
Causes serious eye damage.
May cause damage to organs.
Color Blue
Physical Form Liquid
Solubility Information Miscible with water.
Health Hazard 1 H290-H302+H332-H314-H335-H371
UN Number UN1805
DOT Information Transport Hazard Class: 8; Packing Group: III; Proper Shipping Name: PHOSPHORIC ACID SOLUTION
Chemical Name or Material Bradford Dye Reagent
TSCA No
Recommended Storage Keep cold
6

Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 1X), sterile-filtered

 

EDGE
7

Karl Fischer Aqualine™ Titrant 5, for Karl Fischer Titration By Volumetry, Fisher Chemical

For two-component system. Water equivalent: 5.0mg H2O/mL

Promotions are available
8

Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)

Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.

EDGE
9

Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular, Thermo Scientific Chemicals

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Promotions are available
Color Yellow to Orange
Physical Form Solution
UN Number 2927
Chemical Name or Material Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0
Grade Molecular Biology
Merck Index 14, 7241
Density 1.2800g/mL
Treatment(s) Stabilized
Name Note DNase, RNase and Protease Free
CAS 123-51-3
MDL Number MFCD00133763
Health Hazard 2 Suspected of causing genetic defects, Suspected of damaging the unborn child, Causes damage to organs through prolonged or repeated exposure, Causes severe skin burns and eye damage, May cause drowsiness or dizziness, Suspected of causing cancer, Repeated exposure may cause skin dryness or cracking, Toxic if swallowed, in contact with skin or if inhaled
pH 7.8 to 8.2 (25°C)
Packaging Glass bottle
Health Hazard 1 Germ cell mutagenicity (category 2), Reproductive toxicity (category 2), Specific target organ toxicity after repeated exposure (category 1), Skin corrosion/irritation (category 1), Specific target organ toxicity after single exposure (category 3), Carcinogenicity (category 2), Acute toxicity (category 3)
Molecular Formula C6H6O
Formula Weight 94.11
Specific Gravity 1.28
10

Ethanol, 95% (v/v), Certified AR for Analysis, Fisher Chemical™

CAS: 64-17-5 Molecular Weight (g/mol): 46.07 MDL Number: 3568 InChI Key: LFQSCWFLJHTTHZ-UHFFFAOYSA-N PubChem CID: 702 ChEBI: CHEBI:16236 IUPAC Name: ethanol SMILES: CCO

Promotions are available
PubChem CID 702
CAS 64-17-5
Molecular Weight (g/mol) 46.07
ChEBI CHEBI:16236
MDL Number 3568
SMILES CCO
IUPAC Name ethanol
InChI Key LFQSCWFLJHTTHZ-UHFFFAOYSA-N
11

Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

CAS: 136112-00-0 Molecular Formula: C12H19Cl3O2 Molecular Weight (g/mol): 301.632 MDL Number: MFCD00133763 InChI Key: ZYWFEOZQIUMEGL-UHFFFAOYSA-N Synonym: phenol-chloroform-isoamyl alcohol mixture,chloroform; isoamyl alcohol; phenol,phenol chloroform isoamyl alcohol,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1,phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1,phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer,phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer PubChem CID: 66587205 IUPAC Name: chloroform;3-methylbutan-1-ol;phenol SMILES: CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl

PubChem CID 66587205
CAS 136112-00-0
Molecular Weight (g/mol) 301.632
MDL Number MFCD00133763
SMILES CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl
Synonym phenol-chloroform-isoamyl alcohol mixture,chloroform; isoamyl alcohol; phenol,phenol chloroform isoamyl alcohol,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1,phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1,phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1,phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer,phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
IUPAC Name chloroform;3-methylbutan-1-ol;phenol
InChI Key ZYWFEOZQIUMEGL-UHFFFAOYSA-N
Molecular Formula C12H19Cl3O2
12

Gibco™ PBS, pH 7.2
GREENER_CHOICE

PBS, pH 7.2

EDGE
Dilution 1 X
Content And Storage 15°to 30°C
Additive No Sodium Pyruvate
Form Liquid
Product Type Phosphate Buffered Saline (PBS)
pH 7.2
Shelf Life 36 Month(s)
For Use With (Application) Mammalian Cell Culture
Osmolality 280 to 320 mOsm/kg
13

Thermo Scientific Chemicals MOPS, 1.0M buffer soln., pH 8.0

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

PubChem CID 70807
CAS 1132-61-2
Molecular Weight (g/mol) 209.26
ChEBI CHEBI:44115
MDL Number MFCD00006183
SMILES [O-]S(=O)(=O)CCC[NH+]1CCOCC1
IUPAC Name 3-morpholin-4-ylpropane-1-sulfonic acid
InChI Key DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula C7H15NO4S
14

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.5%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
MDL Number MFCD00004679
SMILES C(C(CO)(CO)N)O
Synonym TRIS
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
15

Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6

For preparation of protein samples for native gel electrophoresis

Color Blue
Physical Form Liquid
pH 8.6
Chemical Name or Material Sample Loading Buffer
Concentration 4X
For Use With (Application) Preparation of protein samples for native gel electrophoresis