Invitrogen™ TRIzol™ Plus RNA Purification Kit

The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast.

Brand:  Invitrogen™ 12183555

Product Code. 12094346

Includes: TRIzol Reagent (100mL); Lysis Buffer (125mL); Wash Buffer I (50mL); Wash Buffer II (15mL); RNase-Free Water (15.5mL); Spin Cartridges with collection tubes (50 each); Collection Tubes (50 each); Recovery Tubes (50 each). Sufficient reagents are provided to perform 50 RNA isolations.

Description

The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).

• Combined TRIzol-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol
• Fewer resources used, less hazardous, less waste

Not high-throughput compatible (Manual)

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: • 100 mL TRIzol Reagent; room temperature
  • 1 PureLink RNA Mini Kit: 125 mL Lysis Buffer; room temperature
  • 50 mL Wash Buffer I; room temperature
  • 15 mL Wash Buffer II; room temperature
  • 15.5 mL RNase-free Water; room temperature
  • 50 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
  
  
• Purification Time: 1 hr.
• Sample Type: Bacteria, Cells, Plant Samples, Tissue, Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 350 μg

• Isolation Technology: Silica Spin Column, Organic Extraction
• Elution Volume: 30 to 300 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁷
  Cells: ≤10⁷
  Plant: ≤10⁷, ≤100 mg
  Tissue: ≤100 mg
  Yeast: ≤10⁷

Safety and Handling

Product Identifier

• TRIzol Plus RNA Purification Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid, Phenol, Ammonium thiocyanate

For Research Use Only. Not for use in diagnostic procedures.