Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Product Code	Brand	Quantity
11866754	Thermo Scientific™ 20339	10 mL
11876754	Thermo Scientific™ 20340	1000 mL

Description

Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples.

Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand binds to the lipid A portion of lipopolysaccharides (LPS), which are the dominant form of endotoxins that affect biological samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be added to cells for various treatment experiments. Detoxi-Gel Resin provides a quick method for removal of endotoxins from samples. It is available in convenient pre-packed columns and two sizes of slurry for use in small-scale or large-scale processes.

Highlights:

• Affinity-based – uses immobilized polymixin B to remove endotoxins by binding to their lipid A domains

• High capacity – 1mL of Detoxi-Gel Resin removes at least 9995 endotoxin units from a 5mL sample containing 10,000 EU of lipopolysaccharide (LPS)

• Reusable – no loss in binding capacity even after 10 regenerations; requires activation and regeneration with 1% sodium deoxycholate (Part No. 89904)

• Testing required – protein recovery is dependent upon protein type and concentration; empirical testing is required to validate the method for particular protein samples

• Versatile – remove endotoxin from protein solutions, cell culture media and pharmacological components

Requires:

Sodium deoxycholate (Part No. 89904) for resin activation and regeneration.

Specifications

• Capacity (Metric): 4000 to 6000 EU Binding per mL Gel
• Product Line: Detoxi-Gel™
• Type: Endotoxin Removing Gel
• Format: Bottle

• Content And Storage: Store at 4°C.
• Target: Endotoxin Removal
• Stationary Phase: Polymixin B
• Column Type: Affinity, Agarose Resin

Safety and Handling

Product Identifier

• Detoxi-Gel Endotoxin Removing Gel

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H350-May cause cancer.
• H360Df-May damage the unborn child. Suspected of damaging fertility.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.