Gibco™ ExpiSf™ CD Medium

Gibco™ ExpiSf™ CD Medium is chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.

Product Code	Brand	Quantity
15879116	Gibco™ A3767801	500 mL
15889116	Gibco™ A3767802	1000 mL
15899116	Gibco™ A3767803	6 x 1000 mL
15809126	Gibco™ A3767804	10 L
15819126	Gibco™ A3767805	20 L

Description

• Delivers high reproducibility and lot-to-lot reliability
• Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
• Supports growth of suspension Sf9 cultures to densities of approximately 2 x 10⁷ cells/mL, with >90% cell viability
• Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
• Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3L in shaker flasks
• Is a ready-to-use formulation, with no supplementation required
• manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP)

ExpiSf™ CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit (Cat. No. A3767806). Sf21 and High Five™ cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf™ CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.

Specifications

• Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
• Format: Liquid
• Product Line: ExpiSf™

• Compatible Cells: Insect Cells
• Shipping Condition: Room Temperature