Cytiva Capto™ Core 700

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Product Code	Brand	Quantity
12633860	Cytiva 17-5481-02	100 mL
12603860	Cytiva 17-5481-01	25 mL

Description

The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography medium (resin).

• For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.
• Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
• Significantly improved productivity and higher flow rates compared with gel filtration methods.
• Straightforward optimization due to flowthrough chromatography and robust performance.
• Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
• Capto Core 700 is composed of a ligand-activated core and inactive shell.
• The inactive shell excludes large molecules (cut off ∼ Mr 700000 [700 kDa]) from entering the core through the pores of the shell.
• These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
• The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core.
• The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations.
• Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.

Specifications

• Buffer: 1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
• Flow Rate: <500 cm/hr.
• pH Range: 2 to 14
• Support Type: Highly Cross-linked Agarose
• For Use With (Application): Intermediate purification and polishing of viruses and other large biomolecules

• Product Type: Capto Core 700 chromato graphy media
• Ligand Type: Octylamine
• Particle Size: 90μm
• Content And Storage: 4° to 30°C
• Matrix: Multimodal Resin

Safety and Handling

Product Identifier

• Capto Core 700, 100 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.