Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Brand:  Cytiva 17-5318-01

Product Code. 10291210

Description

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

• Designed for scaling up purification of histidine-tagged proteins.
• High protein binding capacity and minimal leakage of Ni2+ ions.
• As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
• Available in convenient prepacked HisPrep and HisTrap column formats.
• Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
• The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
• Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
• As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Specifications

• Buffer: 0.1M HCl, 0.1M NaOH, 8M Urea
• Quantity: 25mL
• Height (Metric): 250mm
• Max. Pressure: 1 bar
• Particle Size: 90μm
• Content And Storage: 4° to 30°C

• Product Type: Ni Sepharose 6 FF chromatography media
• Height (English): 9.84 in.
• Flow Rate: 600cm/hr.
• pH Range: 3 to 12
• Support Type: 6% Cross-linked Agarose
• Matrix: Ni-Sepharose

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Ethanol, Nickel