Cytiva GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Product Code	Brand	Quantity
10647455	Cytiva 17-5281-01	5 x 1 mL
11565035	Cytiva 17-5282-02	5 x 5 mL
11713219	Cytiva 17-5282-01	1 x 5 mL

Description

• One-step purification of GST-tagged proteins and other glutathione S-transferases or glutathione-dependent proteins
• Glutathione coupled to Sepharose™ High Performance media
• Column material: polypropylene
• May be used with syringe, peristaltic pump or chromatography system
• Purification process preserves antigenicity and function
• Available as 1mL and 5mL columns

Media

• Average particle size: 34μm
• Ligand: Glutathione
• Matrix: Highly cross-linked 6% agarose
• Binding capacity: >7mg/mL
• pH stability working range: 3 to 12

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Description: GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 34 μm
• Type: Pre-packed Column

• Material: Polypropylene
• Support Type: 4% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• For Use With (Application): Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
• Ligand Type: Glutathione

Safety and Handling

Product Identifier

• GSTrap HP 1 ml, 5 x 1 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : ethanol