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Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Brand: Thermo Scientific™ 25231
Code : PN
Additional Details : Weight : 0.01000kg
Description
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.
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Highlights:
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Large scale FPLC purification of polyhistidine tagged proteins
Specifications
Store at 4°C | |
HisPur™ | |
His-Tagged Protein | |
Metal Chelate, Divalent Cobalt | |
Affinity, Agarose Resin |
Liquid Suspension | |
1 L | |
Agarose | |
Bottle |
For Research Use Only. Not for use in diagnostic procedures.