Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Brand:  Thermo Scientific™ 88831

Product Code. 13464289

Description

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.

Additional Information and Online Ordering

The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.

Highlights:

• High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers

• Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification

• Fast – protocol is completed in 1 hour

• Scalable – process microliter to milliliter sample volumes

• Versatile – purify proteins using native or denaturing conditions

• Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives

• Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)

Specifications

• Content And Storage: Store at 4°C
• Product Line: HisPur™
• Protein Form: Recombinant
• Type: Magnetic Affinity Bead

• Form: Liquid
• Quantity: 2 mL
• Target: His
• Ligand Type: Nickel-NTA

Safety and Handling

Product Identifier

• Ni-NTA Magnetic Beads

Signal Word

• Warning

Hazard Category

• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : sulphuric acid

For Research Use Only. Not for use in diagnostic procedures.