Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Product Code	Brand	Quantity
13484289	Thermo Scientific™ 88832	10 mL
13464289	Thermo Scientific™ 88831	2 mL

Description

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.

Additional Information and Online Ordering

The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.

Highlights:

• High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers

• Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification

• Fast – protocol is completed in 1 hour

• Scalable – process microliter to milliliter sample volumes

• Versatile – purify proteins using native or denaturing conditions

• Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives

• Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)

Specifications

• Content And Storage: Store at 4°C
• Protein Form: Recombinant
• Type: Magnetic Affinity Bead

• Product Line: HisPur™
• Target: His
• Ligand Type: Nickel-NTA

Safety and Handling

Product Identifier

• HisPur Ni-NTA Magnetic Beads

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H228-Flammable solid.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H350-May cause cancer.
• H360-May damage fertility or the unborn child.

Precautionary Statement

• P202-Do not handle until all safety precautions have been read and understood.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol, sulphuric acid

For Research Use Only. Not for use in diagnostic procedures.