Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Product Code	Brand	Diameter (Metric) Inner	Quantity	Flow Rate
12632966	Cytiva 17-3712-05	7 mm	5 x 1 mL	1 to 4 mL/min.
12683466	Cytiva 17-3712-06	16 mm	5 x 5 mL	5 to 20 mL/min.

Description

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

• Ready-to use prepacked with Ni Sepharose excel
• Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
• Made of biocompatible polypropylene and cannot be opened or refilled
• Delivered with stopper on inlet and snap-off end on outlet
• Operated either with peristaltic pump or with chromatography systems such as KTA systems
• Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium

Media

• Average particle size: 90μm
• Matrix: Highly cross-linked agarose, 6%
• pH Stability working range: 2 to 12
• pH Stability cleaning range: 2 to 14
• Flow velocity: 150 cm/h - 600 cm/h⁴

Column:

• Column I.D.: 7 mm

HisTrap excel 1mL column

• Bed Dimensions: 7 × 25mm
• Flow rate: 1 to 4mL/min.

HisTrap excel 5mL column

• Bed Dimensions: 16 × 25mm
• Flow rate: 5 to 20mL/min.

Specifications

• Description: HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
• pH Range: 2 to 12
• Content And Storage: 4°C to 30°C
• Type: Pre-packed Column

• Material: Polypropylene
• Support Type: 6% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : ethanol