Cytiva HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Brand:  Cytiva 17-0409-05

Product Code. 11850073

Description

• Uses different kinds of metal ions
• Binding capacity ∽12mg histidine-tagged protein/mL medium
• Metal ion capacity ∽23μmol Cu²⁺/mL medium
• Prepacked with Chelating Sepharose High Performance media
• Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

Specifications

• Buffer: 6M Guanidine Hydrochloride, 8M Urea
• Bed Volume: 5mL
• Height (English): 0.98 in.
• Flow Rate: <20 mL/min.
• Ligand Type: Iminodiacetic Acid
• pH Range: 3 to 13
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Affinity Column

• Product Type: HiTrap chelating HP column
• Quantity: 100 x 5 mL
• Height (Metric): 25mm
• Material: Iminiodiacetic acid chelating group
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Content And Storage: 4° to 30°C
• For Use With (Application): IMAC and subsequent purification of Polyhistidine tagged Proteins