Thermo Scientific™ Imject™ BSA (in MES Buffer)

Easily prepare immunogens from carboxyl-containing peptide antigens with bovine serum albumin (BSA) and different conjugation chemistries.

Brand:  Thermo Scientific™ 77171

Product Code. 11894071

Description

Thermo Scientific™ Imject Bovine Serum Albumin (BSA) is offered lyophilized in PBS or MES buffer for use in different conjugation chemistries and as a complete kit to prepare immunogens from carboxyl-containing peptide antigens.

Imject BSA is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. The large, single polypeptide protein contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers.

Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of BSA carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

• Convenient packages – choose lyophilized BSA or complete EDC conjugation kit

• High-yield conjugation – each molecule of 59 lysine residues, 30 to 35 of which have primary amines that are capable of reacting with a conjugation reagent

• Validated quality – purified BSA (Fraction V) is available lyophilized and stabilized in PBS or MES buffer, ready for use in glutaraldehyde, NHS-ester or EDC crosslinking methods

• Compatible solubility – numerous carboxyl groups account for the net negative charge (pI 5.1) of BSA and its high solubility, allowing its use in a broad range of conjugation conditions

• Immunogenic – at 67kDa, BSA is sufficiently large to function as the primary immunogen or it can be used as an irrelevant protein carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten

Includes:

Kit contains lyophilized BSA (in MES buffer), EDC crosslinker, buffers and desalting to easily prepare ready-to-use immunogens for injection

Specifications

• Content And Storage: 4°C
• Description: Imject Bovine Serum Albumin (in MES buffer)
• Quantity: 2 mg
• Product Line: Imject™

Safety and Handling

Product Identifier

• Imject BSA (in MES Buffer)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 4-Morpholineethanesulfonic acid, hydrate

For Research Use Only. Not for use in diagnostic procedures.