Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit

Offers ability to perform both a gel extraction or PCR purification with single kit.

Brand:  Invitrogen™ K220001

Product Code. 10606845

Includes: The PureLink Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 50 preps.

Description

• Offers ability to perform both a gel extraction or PCR purification with single kit
• Simple procedure uses silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes
• Provides all components to perform rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fragments >100 bp in less than 10 minutes
• Isolated DNA using PureLink kit is free of proteins, dye, and agarose, and is ready-to-use for variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling
• Contains all buffers fromPureLink Quick Gel extraction kit and PCR binding buffer from PureLink PCR Purification kit with Invitrogen lidded column
• Purification time is 20 minutes

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 2 x 90 mL Gel Solubilization Buffer L3; room temperature
  • 15 mL Binding Buffer B2; room temperature
  • 16 mL Wash Buffer W1; room temperature
  • 15 mL Elution Buffer E1; room temperature
  • 50 PureLink Clean-up Spin Columns with Collection Tubes; room temperature
  • 50 PureLink Elution Tubes; room temperature
  
  
• Sample Type: Linear DNA fragments from: Agarose Gels, PCR Purification, Restriction Enzyme Reactions, PCR Products
• Elution Volume: 50 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Label or Dye: Ethidium Bromide, SYBR Safe
• Shipping Condition: Room Temperature
• Yield: 40 μg (Binding capacity)

• Isolation Technology: Silica Spin Column
• Scale: Mini
• Final Product Type: DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Test Time: Up to 30 min.
• Starting Material Amount: Agarose Gel: ≤400 mg
  PCR Product: ≤100 μL, or ≤40 μg dsDNA

Safety and Handling

Product Identifier

• PureLink Quick Gel Extraction and PCR Purification Combo Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid, Guanidine hydrochloride

For Research Use Only. Not for use in diagnostic procedures.