Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Brand:  Cytiva 28-9187-78

Product Code. 10075174

Description

• Prepacked with Dextrin Sepharose tagged with maltose binding protein (MBP).
• Binding capacity is fusion protein dependent but can be as much as 16 mg/ml media
• Maximum pressure: 3 bar
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
• 1 and 5mL polypropylene columns can be used either manually using a syringe or on an instrument

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: >7

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Buffer: Aqueous Buffer
• Bed Volume: 1mL
• Diameter (Metric) Inner: 7mm
• Height (English): 0.98 in.
• Flow Rate: <4 mL/min.
• Ligand Type: Dextrin
• pH Range: >7
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Ion Exchange Column

• Product Type: Dextrin Sepharose HP prepacked column
• Diameter (English) Inner: 0.27 in.
• Quantity: 5 x 1mL
• Height (Metric): 25mm
• Material: Polypropylene
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Content And Storage: 2° to 8°C
• For Use With (Application): Purification of MBP tagged Proteins

Safety and Handling

Product Identifier

• MBPTrap HP

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol