Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Product Code	Brand	Quantity	Flow Rate
10097924	Cytiva 28-9187-79	1 x 5mL	<20 mL/min.
10075174	Cytiva 28-9187-78	5 x 1mL	<4 mL/min.
11500664	Cytiva 28-9187-80	5 x 5mL	<20 mL/min.

Description

• Prepacked with Dextrin Sepharose tagged with maltose binding protein (MBP).
• Binding capacity is fusion protein dependent but can be as much as 16 mg/ml media
• Maximum pressure: 3 bar
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
• 1 and 5mL polypropylene columns can be used either manually using a syringe or on an instrument

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: >7

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Buffer: Aqueous Buffer
• Bed Volume: 1mL
• Height (Metric): 25mm
• Ligand Type: Dextrin
• pH Range: >7
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Ion Exchange Column

• Product Type: Dextrin Sepharose HP prepacked column
• Height (English): 0.98 in.
• Material: Polypropylene
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Content And Storage: 2° to 8°C
• For Use With (Application): Purification of MBP tagged Proteins

Safety and Handling

Product Identifier

• MBPTrap HP, 5 ml, 1 x 5 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 2

Hazard Statement

• H225-Highly flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : ethanol