Gibco™ Medium 199 (2X)

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Brand:  Gibco™ 21157029

Product Code. 11590556

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With: L-glutamine

Without: HEPES, Phenol Red

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This Medium 199 is made with Earle's salts.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

• Content And Storage: Storage conditions: 2 – 8°C. Protect from light.
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Concentration: 2 X
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Phenol Red
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Type: Mammalian Cells
• Culture Type: Mammalian Cell Culture
• Product Type: Medium 199
• With Additives: Glutamine, Earle's Balanced Salt Solution (EBSS)
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 250 mL

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.