Cytiva NHS Mag Sepharose™

NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques

Product Code	Brand	Binding Capacity	For Use With (Application)	Quantity
10636375	Cytiva 28-9513-80	8 to 14μM/mL	Designed for covalent coupling of any Protein or biomolecule containing a free Amine group and is used for various types of pull down experiments, for biomarker research	4 x 500 μL
10371315	Cytiva 28-9440-09	8 to 14μM/mL Gel	For pull-down techniques enabling rapid capture and enrichment of selected Proteins based on affinity	500 μL

Description

NHS Mag Sepharose are magnetic beads designed for pull-down techniques enabling rapid capture and enrichment of selected proteins based on affinity. An antibody or other protein with desired affinity is covalently coupled to the medium via primary amines for the purpose of capture of target proteins of interest.

• Visible and dense Sepharose based magnetic beads with N-hydroxysuccinimide (NHS) ligand, easy to spot and collect bound target protein
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
• Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
• Optimized capacity for enrichment/pull-down of target proteins - only low amounts of bound capture molecule
• Flexible protocols with elution conditions optimized for downstream applications such as electrophoresis and MS

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: N-hydroxysuccinimide
Ligand density: 8-14μmol/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% 2-propanol
Storage temperature: 4°C to 8°C

Specifications

• Bead Type: NHS Mag Sepharose
• Matrix: Paramagnetic spherical, highly cross linked agarose particles
• Content And Storage: 4°C to 8°C

• Ligand Concentration: 8 to 14μM/mL
• Particle Size: 37 to 100 μm
• Ligand: N-hydroxysuccinimide

Safety and Handling

Signal Word

• Danger||

Hazard Category

• Flammable liquid Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• UNKNOWN AQUATIC-Contains 15% of components with unknown hazards to the aquatic environment.
• UNKNOWN ACUTE-100 per cent of the mixture consists of component(s) of unknown acute toxicity.