Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Brand:  Cytiva 17-3712-02

Product Code. 12664557

Description

Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
• Increase target protein yield and decrease degradation through reduced and simplified sample handling.
• Choose between several formats for screening and preparative purification of histidine-tagged proteins.
• Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
• Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Specifications

• Buffer: 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
• Quantity: 100mL
• pH Range: 2 to 12
• Support Type: 6% Highly Cross-linked Agarose
• For Use With (Application): Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)

• Product Type: Ni Sepharose excel chromato graphy media
• Flow Rate: 150 to 600cm/hr.
• Particle Size: 90μm
• Content And Storage: 4° to 30°C
• Matrix: Ni-Sepharose

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains nickel. May produce an allergic reaction.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P235-Keep cool.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : nickel, ethanol