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BD Biosciences™ NKTEST™ (CE/IVD)

For quantitative determination of the cytotoxic activity of human natural killer (NK) cells.

£583.70 - £2433.90

Specifications

Content And Storage Reagent A (complete medium) and reagent C (DNA staining solution) must be stored at 2-8°C (in refrigerator). Store Reagent B (Interleukin-2) at >-15°C. The K562 target cells have to be stored at - 70°C
Detection Method Flow Cytometry, Stain
Stability Cells are stable for 12 weeks showing a viability >90%.
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Description

Description

  • This reagent kit allows the quantitative determination of the cytotoxic activity of human natural killer (NK) cells.
  • Contains cryopreserved prestained K562 target cells, complete medium and a DNA-staining reagent.
  • The K562 target cells are labelled with a lipohilic green fluorescent membrane dye in order to discriminate effector and target cells.
  • After the incubation period in the cytotoxicity assay, killed target cells are identified by a DNA-stain, which penetrates the dead cells and specifically stains their nuclei.
  • In this manner, the percentage of target cells killed by effector NK cells can be determined.
Specifications

Specifications

Reagent A (complete medium) and reagent C (DNA staining solution) must be stored at 2-8°C (in refrigerator). Store Reagent B (Interleukin-2) at >-15°C. The K562 target cells have to be stored at - 70°C
Flow Cytometry, Stain
Cells are stable for 12 weeks showing a viability >90%.
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