Pierce™ Pierce™ Ni-NTA Magnetic Agarose Beads

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins

Brand:  Pierce™ 78606

Product Code. 15666144

Description

Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.

• High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
• High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
• Low nonspecific binding— optimized purification protocol results in better tagged-protein purification
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

Applications:

• Protein purification and isolation
• High throughput protein purification using automated magnetic particle processor

Target molecule: His-Tag

Specifications

• Content And Storage: 5 mL settled resin. Supplied as 20 mL of 25% v/v suspension in 20% ethanol.
  
  Store at 2–8°C.
• Form: Liquid Suspension
• Quantity: 20 mL
• Type: Magnetic Agarose Bead
• Format: Bottle

• Shipping Condition: Wet Ice
• Product Line: Pierce™
• Protein Form: Recombinant
• Stationary Phase: Metal Chelate, Divalent Nickel

Safety and Handling

Product Identifier

• Pierce Ni-NTA Magnetic Agaros

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Carcinogenicity Category 2
• Flammable liquid Category 3
• Respiratory sensitiser Category 1B
• Skin sensitiser Category 1B

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H351-Suspected of causing cancer.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P403+P235-Store in a well-ventilated place. Keep cool.

Supplemental information

• MIXTURE LIST-Contains : ethyl alcohol

For Research Use Only. Not for use in diagnostic procedures.