Pierce™ Pierce™ Ni-NTA Magnetic Agarose Beads

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins

Product Code	Brand	Quantity
15656144	Pierce™ 78605	4 mL
15666144	Pierce™ 78606	20 mL

Description

Product features: 
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein. 

• High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
• High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
• Low nonspecific binding—  optimized purification protocol results in  better tagged-protein purification
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

Applications:

• Protein purification and isolation 
• High throughput protein purification using automated magnetic particle processor

Specifications

• Shipping Condition: Wet Ice
• Product Line: Pierce™
• Type: Magnetic Agarose Bead
• Format: Bottle

• Form: Liquid Suspension
• Protein Form: Recombinant
• Stationary Phase: Metal Chelate, Divalent Nickel

For Research Use Only. Not for use in diagnostic procedures.