Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Product Code	Brand	Quantity
12146490	Thermo Scientific™ 88700	5 kU
12391963	Thermo Scientific™ 88702	100 kU
12156490	Thermo Scientific™ 88701	25 kU

Description

Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.

Highlights:

• Broad spectrum – degrades all forms of DNA and RNA
• Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE
• Robust activity – 100-fold greater specific activity than DNase I
• Versatile – can be used with a wide variety of cell lysis reagents

Recommended for:

Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing

Storage Requirements

Store in a cool, dry, well-ventilated area, protected from direct sunlight

Specifications

• Product Type: Cell Lysis Enzyme
• Form: Liquid
• Reagent Type: Enzyme for Cell Lysis

• Content And Storage: Store in a cool, dry, well-ventilated area, protected from direct sunlight.
• Product Line: Pierce™
• Enzyme: Nuclease

For Research Use Only. Not for use in diagnostic procedures.