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R&D Systems™ Recombinant Human R-Spondin 1 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 4645-RS-100
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Description
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
Q2MKA7 | |
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
25.6 kDa | |
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein Ser21-Ala263 | |
RUO | |
RSPO1 | |
R-Spondin 1 | |
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Bioactivity | |
284654 | |
100 μg | |
Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution | |
<0.10 EU per 1 μg of the protein by the LAL method. | |
Unconjugated | |
Human | |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
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