Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Product Code	Brand	Binding Capacity	Quantity
10313350	Cytiva 17-1279-02	50 mg Human lgG/mL	25 mL
10302870	Cytiva 17-1279-01	50 mg Human lgG/mL medium2	5 mL
10117549	Cytiva 17-1279-03	50 mg Human lgG/mL medium2	200 mL

Description

• Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
• High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
• No mammalian components involved during manufacturing process
• Matrix: 4% cross-linked agarose
• Average particle size: 90μm (d50V)
• Ligand: 6mg rProtein A/mL medium (E. coli)
• Binding capacity: ∽50mg human IgG/mL media
• pH stability working range: 3 to 10
• Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

Specifications

• For Use With (Application): For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
• Storage Requirements: 2°C to 8°C
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• pH Range: 2 to 9
• Ligand Type: Recombinant Protein A (E. coli)

• Stability: Stable in aqueous Buffers commonly used in Protein A Chromatography
• Product Type: rProtein A Sepharose Fast Flow Media
• Particle Size: 60 to 165 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol

Safety and Handling

Product Identifier

• rProtein A Sepharose

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.