Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Brand:  Cytiva 17-1279-01

Product Code. 10302870

Description

• Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
• High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
• No mammalian components involved during manufacturing process
• Matrix: 4% cross-linked agarose
• Average particle size: 90μm (d50V)
• Ligand: 6mg rProtein A/mL medium (E. coli)
• Binding capacity: ∽50mg human IgG/mL media
• pH stability working range: 3 to 10
• Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

Specifications

• Quantity: 5 mL
• For Use With (Application): For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
• Binding Capacity: 50 mg Human lgG/mL medium2
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• pH Range: 2 to 9
• Ligand Type: Recombinant Protein A (E. coli)

• Stability: Stable in aqueous Buffers commonly used in Protein A Chromatography
• Storage Requirements: 2°C to 8°C
• Product Type: rProtein A Sepharose Fast Flow Media
• Particle Size: 60 to 165 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 2

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : ethanol