Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

in vitro synthesis of large amounts of capped RNA

Brand:  Invitrogen™ AM1344

Product Code. 10438644

Description

mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

• RNA Yield: 20-30μg
• Include cap analog [m⁷G(5′)ppp(5′)G] in ultra high-yield transcription reaction
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
• For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
• High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
• RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

Specifications

• Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Format: Kit
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• Product Line: Ambion™, mMESSAGE mMACHINE™
• Quantity: 25 Reactions
• Shipping Condition: Dry Ice
• Starting Material: PCR Products, Linearized DNA plasmid

• Final Product Type: Synthetic mRNA, mRNA
• Sample Type: mRNA, Synthetic mRNA
• No. of Reactions: 25 Reactions
• Product Type: T7 In Vitro Transcription kit
• Promoter: T7
• Yield: 20-30 μg

Safety and Handling

Product Identifier

• mMESSAGE mMACHINE T7 Transcription Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 1B
• Skin corrosion/irritation Category 2
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H351-Suspected of causing cancer.
• H360-May damage fertility or the unborn child.
• H373-May cause damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P314-Get medical advice/attention if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : lithium chloride, formamide

For Research Use Only. Not for use in diagnostic procedures.