IBA Lifesciences Strep-Tactin™ TACS Agarose Column

Gravity flow column suitable for traceless affinity cell and exosome isolation

Brand:  IBA Lifesciences 6-6310-001

Product Code. 17227750

Description

• Strep-Tactin™ TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
• A special crosslinking permits the use for cell isolation and depletion approaches.
• They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS™/Nano-TACS™).
• In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
• After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
• This way the cells are eluted ready for further downstream applications.
• The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes).
  
  
• Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding).
• This makes Strep-Tactin™ TACS Agarose columns also suitable for negative cell selection or depletion approaches.

Specifications

• Capacity (Metric): 1 mL
• Formulation: Pre-packed in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide
• Support Type: Cross-linked agarose
• For Use With (Application): Affinity Chromatographic Cell or Exosome Isolation

For Research Use Only.