Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Brand:  Thermo Scientific™ F170S

Product Code. 15264756

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

• Direct PCR reduces time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
• Specially engineered Phire DNA polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Content And Storage: • 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
  • Dilution Buffer, 5 mL
  • DNARelease Additive, 3 x 100 μL
  • 25 μM Control Primer Mix, 40 μL
  • Nuclease-free water, 2 x 1.25 mL
  • 50 μg O'GeneRuler Express DNA Ladder
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Master Mix
• For Use With (Application): Genotyping, Hot-start PCR
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Tissue

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA Polymerase
• Includes Label or Dye: Yes
• Shipping Condition: Wet or Dry Ice
• Concentration: 2X
• No. of Reactions: 250 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

Safety and Handling

Product Identifier

• Phire Tissue Direct PCR Master Mix/DNARelease Additive

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachium album serine

For Research Use Only. Not for use in diagnostic procedures.