Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Brand:  Cytiva 27-0846-01

Product Code. 10218854

Description

• Supplied as lyophilized powder
• Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
• Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
• Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

Specifications

• Buffer: Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄ pH 7.3
• Product Type: Thrombin Protease Fusion Protein
• Quantity: 500U
• For Use With (Application): Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours

Safety and Handling

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : thrombin