Vimentin Monoclonal Antibody (RV203), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA106908

Product Code. 11578832

Description

MA1-06908 detects vimentin in human, rat, goat, canine, hamster and swine samples. MA1-06908 has sucessfully been used in Western blotting, immunocytochemistry and immunohistochemistry (paraffin) procedures. By Western blot it detects a ∽57 kDa band representing vimentin. The MA1-06908 immunogen is cytoskeletal vimentin extract of calf lens. Store at 4oC, or in small aliquots at -20oC.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P02543, P08670, P20152, P31000, P48616, P48670
• Gene Symbols: Vim
• Immunogen: Cytoskeletal vimentin extract of calf lens.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Canine, Goat, Hamster, Human, Mouse, Porcine, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: RV203
• Conjugate: Unconjugated
• Gene: Vim
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 100522394, 100689099, 102182515, 22352, 280955, 477991, 7431, 81818
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid